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Reporter genes are attached to a regulatory sequence of a gene of interest. When expressed by the host organism, they generate measurable, easily identifiable characteristics that can be used as an indication of whether a gene has been taken up or expressed.
Cell Biolabs' QuickTiter™ AAV Quantitation Kit provides an ultra-fast 45-60 minute procedure that measures physical titer of unpurified AAV-2 or AAV-DJ, or of purified virus from any AAV serotype, via the viral nucleic acid content. Quantitation is performed on a 96-well fluorescence based plate reader. The kit provides sufficient reagents for 20 assays.
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IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.
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